Mass Spectrometer (Exploris 480)

DTU Bioengineering is looking to acquire a new mass spectrometer and two LC systems to expand their capacity for proteomic samples in the field of spatial proteomics all the way down to single-cell level. The instruments will be used for running large-scale spatial proteomics experiments and will be incorporated into the DTU Proteomics Core. The DTU Proteomics Core at DTU Bioengineering houses five Orbitrap mass spectrometers. With the ambition of expanding in the field of spatial proteomics, the facility has a need to increase their current instrument capacity. Currently the most used instrument is the Orbitrap Exploris 480 instrument, which can’t keep up with the increasing demands. It is therefore vital that the DTU Proteomics Core obtains a duplicate setup, to expand their resources to two Orbitrap Exploris 480s and two matching LC systems. The duplicate setup is specifically needed as expansion into the field of spatial proteomics will result in larger sample sets. This will block the instrument for other smaller projects for weeks to months. The duplicate setup will therefore provide capacity and flexibility of the exact same analysis on either of the two instruments. The DTU Proteomics Core needs the mass spectrometer to fulfil all the following criteria: - Robust instrumentation - Resolving power in the low m/z range required for running multiplexed analysis with TMT-labelling with 18-plex and 35-plex methods - High resolution settings of 120,000 and 240,000 for single-cell analysis - Orthogonal separation for low-input samples and samples with matrix interference